Is the DVD drive Sata or IDE, And does the cd1.sys file support DVD drives. Also are you using any adapters between the motherboard and the dvd drive, perhaps an IDE to sata adapter. You say the dos 6.22 disk works .
You could rename Autoexec.bat and config.sys then boot from the 6.22 disk and copy the config.sys and autoexec.bat files from the disk to the hard drive and test the computer boots and reads disks. Then add in the emm386 lines extra until it stops working.